What is the recommended reagent formulation for conjugation?
Carrier proteins (e.g. BSA and gelatin) and buffer additives such as glycerol, detergents, primary amines (e.g. Tris, histidine) can interfere with protein conjugation. Buffer components such as azide and glycerol can be removed by buffer exchange. Ideally reagents should be provided lyophilized or in PBS at stock concentrations of 1-2mg/mL. Stock concentrations of 0.5-5mg/mL are generally acceptable.
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